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. 2003 Jun;14(6):2583–2591. doi: 10.1091/mbc.E02-09-0621

Figure 6.

Figure 6.

Cyclin D1 gene promoter regulation by estradiol-induced PI3K pathway in Hep G2 cells. Luciferase assay detection on HepG2 cells transfected with pXP2-D1–2966-luciferase (a), pXP2-D1Δ-254-luciferase (-254) (b), and pXP2-D1Δ-20-luciferase (-20) (c). After transfection, cells were treated 6 h with vehicle (C) or E2 (10 nM) in the presence or absence of PI3K inhibitors Ly 294002 (Ly) or wortmannin (W) (10 μM each) or pure antiestrogen ICI 182,780 (ICI) (1 μM) (a and b). Data are expressed as percentage variation with respect to the controls and are the means ± SD of four independent experiments. *p < 0.001 compared with respective control values (C), determined using Student's t test. °p < 0.001 compared with respective estradiol values (E2), determined using Student's t test.