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Northern blot analysis of the wild type cultured at pHs 3.0 and 8.0. Total RNA (10 μg) isolated from the wild-type strain cultured for 7 days at pHs 3.0 (lane 1) and 8.0 (lane 2) was separated by electrophoresis in 1.2% agarose-formaldehyde gels, transferred to a nylon membrane, and hybridized with a 32P-labeled PAC1-specific probe (A) and 32P-labeled β-tubulin-specific probe (B).
