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. 2003 Sep;69(9):5222–5227. doi: 10.1128/AEM.69.9.5222-5227.2003

TABLE 3.

Comparison of FB1 production and relative FUM1 expression by wild-type, PAC2A, and PAC2A-C strains

Strain FB1 production (μg of FB1/ml) and FUM1 expression (relative to wild type) at:
pH 4.5
pH 8.4
FB1a FUM1b FB1a FUM1b
Wild type 20 ± 0 10 (8.0-13) NDc 1.0 (0.9-1.1)
PAC2A 25 ± 2 140 (120-150) 10 ± 2 48 (43-52)
PAC2A-C 20 ± 2 39 (37-43) ND 4.3 (3.9-4.7)
a

Data are presented for results obtained 48 h after resuspension. Values are means of three replicates ± standard error.

b

FUM1 expression was evaluated by real-time PCR 36 h after resuspension, normalized to TUB2 expression, and quantified relative to wild-type FUM1 expression at pH 8.4. FUM1 expression data represent the results of a single experiment (repeated three times with similar results). FUM1 expression was calculated as 2−ΔΔCt, with the range (in parentheses) calculated as 2−ΔΔCt+ s− 2−ΔΔCt−s, where s = the standard deviation of the ΔΔCt value.

c

ND, none detected.