TABLE 3.

Comparison of FB₁ production and relative FUM1 expression by wild-type, PAC2A, and PAC2A-C strains

Strain	FB1 production (μg of FB1/ml) and FUM1 expression (relative to wild type) at:
	pH 4.5		pH 8.4
		FB1a	FUM1b	FB1a	FUM1b
Wild type	20 ± 0	10 (8.0-13)	NDc	1.0 (0.9-1.1)
PAC2A	25 ± 2	140 (120-150)	10 ± 2	48 (43-52)
PAC2A-C	20 ± 2	39 (37-43)	ND	4.3 (3.9-4.7)

^aData are presented for results obtained 48 h after resuspension. Values are means of three replicates ± standard error.

^bFUM1 expression was evaluated by real-time PCR 36 h after resuspension, normalized to TUB2 expression, and quantified relative to wild-type FUM1 expression at pH 8.4. FUM1 expression data represent the results of a single experiment (repeated three times with similar results). FUM1 expression was calculated as 2^−ΔΔCt, with the range (in parentheses) calculated as 2^{−ΔΔCt+ s}− 2^{−ΔΔCt−s}, where s = the standard deviation of the ΔΔCt value.

^cND, none detected.