Figure 3.

HPTLC of lipid extracts from wild-type or Acact^−/− peritoneal macrophages demonstrating reduced macrophage cholesterol ester accumulation in Acact^−/− mice. Macrophages were isolated from male mice and incubated with acetylated LDL (50 μg/ml) for 36 h. Lipid extracts were pooled and analyzed by HPTLC (28). Equivalent amounts of lipid extract (from ≈3 μg of protein homogenate) were loaded in each lane; each lane represents lipids from four mice.