TABLE 2.
Anaerobic growth of R. palustris clones
| Genotype | Clonea | Growth onb
|
Utilization of 3-chlorobenzoatee
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| LCMPY
|
Malate
|
BA
|
|||||||
| Culture densityc | Growth rated | Culture densityc | Growth rated | Culture densityc | Growth rated | With benzoate | Without benzoate | ||
| A | 3-15 | + | 6.5 (0.5) | + | NC | − | NG | − | − |
| 3-52 | + | 6.8 (0.8) | + | NC | − | NG | − | − | |
| B | 3-50 | +++ | 13.2 (0.6) | +++ | 12.8 (0.8) | − | NG | − | − |
| 3-55 | +++ | 13.0 (1.0) | +++ | 13.3 (0.8) | − | NG | − | − | |
| C | 3-16 | +++ | 7.8 (0.3) | + | NC | − | NG | − | − |
| 3-57 | +++ | 7.8 (0.3) | + | NC | − | NG | − | − | |
| D | 3-37 | +++ | 7.0 (0.5) | +++ | 13.2 (0.8) | ++ | 18.7 (1.3) | + | − |
| 3-59 | +++ | 7.3 (0.8) | +++ | 13.8 (0.6) | ++ | 19.2 (1.3) | + | − | |
a
Representatives of each genotype that originated from sampling location 3 were used.
b
The media used were as follows: LCMPY, LCM medium containing a mixture of 0.3% peptone and 0.3% yeast extract; malate, LCM medium containing 15 mM malate; BA, LCM medium containing 2 mM benzoate.
c
The optical densities at 660 nm after 2 weeks were as follows: +++, >1.0; ++, 0.5 to 1.0; +, 0.1 to 0.5; and −,<0.1.
d
Doubling time (in hours). The data are averages of three measurements. Standard deviations are indicated in parentheses. NC, not calculated due to the low growth rate; NG, no growth.
e
Utilization of 1 mM 3-chlorobenzoate in the presence or in the absence of 1 mM benzoate.