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. 2007 Aug 29;2(8):e801. doi: 10.1371/journal.pone.0000801

Figure 1. Break light Dam activity assay.

Figure 1

The fluorescence of the hemimethylated substrate oligonucleotide 1 is internally quenched by the dabcyl group. It is a substrate for Dam and yields the fully methylated product 2, which is rapidly cleaved by DpnI, thus forming fluorescent oligonucleotide 3.