TABLE 2.
Primers used in different combinations to develop the best PCR assay for detection of C. jejuni, C. coli, and C. lari
| Target | Primer (sequence) | Reference |
|---|---|---|
| 23S | Therm1 (5′-TATTCCAATACCAACATTAGT) | 6 |
| Therm4 (5′-CTTCGCTAATGCTAACCC) | ||
| 16S | 6-1 (5′-GTCGAACGATGAAGCTTCTA) | 5 |
| 18-1 (5′-TTCCTTAGGTACCGTCAGAA) | ||
| 16S | CF1 (5′-GGAAGGATGACACTTTTCGGAGCG) | 28 |
| CR2 (5′-TCGCGGTATTGCGTCTCATTGTATATGC) | ||
| 16S | C442 (5′-GGAGGATGACACTTTTCGGAGC) | 29 |
| C490 (5′-ATTACTGAGATGACTAGCACCCC) | ||
| 16S | OT1559 (5′-CTGCTTAACACAAGTTGAGTAGG) | 25 |
| 16S | 18-1rev (5′-TTCTGACGGTACCTAAGGAA) | This study |
| 16S | CF1rev (5′-CGCTCCGAAAAGTGTCATCCTCC) | This study |
| 16S | C490rev (5′-GGGGTGGCTAGCCATCTCAGTATT) | This study |
| 16S | JCL-1 (5′-ATAGTTAATCTGCCCTACACAA) | This study |
| 16S | JCL-2 (5′-TCCTTTTCTTAGGGAAGAATTC) | This study |
| 16S | JCL-3 (5′-CGTCAGAATTCTTCCCTAAG) | This study |
| 16S | JCL-4 (5′-AGTTTAGTATTCCGGCTTCGA) | This study |
| 16S | JCL-5 (5′-GATTCCACTGTGGACGGTAA) | This study |
| IACa | CCL-f (5′-OT1559-GGTTCATGAGGACACCTGAGTT) | This study |
| CCR-r (5′-18-1-TATACACTCTCATCCCTCCAAC) | This study |
a
IAC, primers used for construction of the internal amplification control.