TABLE 4.
Results from the preliminary screening of 26 primer combinations against DNA from 18 isolates and strains
| Primer pair | Screening results for:
|
||||||
|---|---|---|---|---|---|---|---|
| Inclusivity (no. of
strains)
|
Exclusivity (no. of strains)
|
||||||
| C. jejuni (n = 1) | C. coli (n = 1) | C. lari (n = 3) | No. of positive target strains/total true positive (%) | C. upsaliensis (n = 5) | C. helveticus (n = 8) | No. of negative nontarget strains/total true negative (%) | |
| 6-1 + 18-1 | 1 | 1 | 3 | 5/5 (100) | 5 | 8 | 0/13 (0) |
| CF1 + CR2 | 1 | 0 | 3 | 4/5 (80) | 4 | 8 | 1/13 (8) |
| C442 + C490 | 1 | 0 | 3 | 4/5 (80) | 5 | 8 | 0/13 (0) |
| 18-1rev + CR2 | 1 | 0 | 3 | 4/5 (80) | 5 | 8 | 0/13 (0) |
| 18-1rev + C490 | 1 | 0 | 3 | 4/5 (80) | 5 | 8 | 0/13 (0) |
| OT1559 + 18-1a | 1 | 1 | 3 | 5/5 (100) | 1 | 0 | 12/13 (92) |
| OT1559 + C490 | 1 | 0 | 3 | 4/5 (80) | 1 | 1 | 11/13 (85) |
| OT1559 + CR2 | 1 | 0 | 3 | 4/5 (80) | 1 | 1 | 11/13 (85) |
| 6-1 + CR2 | 1 | 0 | 3 | 4/5 (80) | 5 | 8 | 0/13 (0) |
| JCL-1 + JCL-3 | 1 | 1 | 3 | 5/5 (100) | 2 | 6 | 5/13 (39) |
| JCL-1 + JCL-4 | 0 | 0 | 0 | 0/5 (0) | 0 | 0 | 13/13 (100) |
| JCL-1 + JCL-5 | 0 | 0 | 0 | 0/5 (0) | 0 | 0 | 13/13 (100) |
| JCL-2 + JCL-4 | 1 | 0 | 2 | 3/5 (60) | 0 | 1 | 12/13 (92) |
| JCL-2 + JCL-5 | 0 | 0 | 0 | 0/5 (0) | 0 | 1 | 12/13 (92) |
| OT1559 + JCL3 | 1 | 1 | 3 | 5/5 (100) | 2 | 5 | 6/13 (46) |
| OT1559 + JCL4 | 0 | 0 | 0 | 0/5 (0) | 0 | 0 | 13/13 (100) |
| OT1559 + JCL5 | 0 | 0 | 0 | 0/5 (0) | 0 | 1 | 12/13 (92) |
| JCL-1 + 18-1 | 1 | 1 | 3 | 5/5 (100) | 2 | 6 | 5/13 (39) |
| C490rev + JCL-4 | 1 | 0 | 3 | 4/5 (80) | 4 | 7 | 2/13 (15) |
| C490rev + JCL-5 | 1 | 0 | 3 | 4/5 (80) | 2 | 2 | 9/13 (69) |
| 18-1rev + JCL-4 | 0 | 0 | 0 | 0/5 (0) | 1 | 1 | 11/13 (85) |
| 18-1rev + JCL-5 | 0 | 0 | 1 | 1/5 (20) | 1 | 2 | 10/13 (77) |
| 6-1 + CF1rev | 1 | 0 | 0 | 1/5 (20) | 1 | 8 | 4/13 (31) |
| JCL-1 + CF1rev | 1 | 1 | 3 | 5/5 (100) | 4 | 8 | 1/13 (8) |
| OT1559 + CF1rev | 1 | 1 | 3 | 5/5 (100) | 2 | 4 | 7/13 (54) |
| C490 + CR2 | 0 | 0 | 0 | 0/5 (0) | 0 | 0 | 13/13 (100) |
a
Note that the OT1559 + 18-1 primer combination was the most selective new combination and was chosen as the final standard test.