TABLE 2.

16S rDNA primer pairs generated for qPCR analysis

Targeted group and product size (nucleotides)	Group most closely affiliated with	Probe name/sequence	Temp (°C)	No. of matches in RDP
I (83)	α-Proteobacteria	GIF/forward primer (5′-3′) AACACCAGTGGCGAAGG	59.61	11
		GIR/reverse primer 2 (3′-5′) GAGCAAACAGGATTAGATACCC	60.81	17
II (171)	β-Proteobacteria	GIIF/forward primer 2 (5′-3′) CGGYAGAGGGGGATGGAA	62.18	0
		GIIR/reverse primer 2 (3′-5′) CCCTAAACGATGTCAACTGG	60.4	0
III (143)	γ-Proteobacteira	GIIIF/forward primer 2 (5′-3′) GAAATGCGTAGAGATCGGGAG	62.57	0
		GIIIR/reverse primer 2 (5′-3′) ACRTCCAGTTCGCATCGTTTAGG	62.77	133
IV (86)	Cyanobacteria	GIVF/forward primer F1(5′-3′) CCWGTAGTCCTAGCCGTAA	60.16	0
		GIVR/reverse primer 2 (3′-5′) CTAACGCGTTAAGTATCCCG	60.4	208