TABLE 2.
Difference alignment of the target region of the 16S rRNA for LKC-specific probes
| Probe and target | Target sequencea |
|---|---|
| LKC59 probe sequence (5′-3′) | TCCTCTCATCGTTCGACT |
| Target sequence | AGUCGAACGAUGAGAGGA |
| LKC group I clonesb | ------------------ |
| Uncultured Cesspool Cave clone CC-4 (AF207530) | -------------U---- |
| Uncultured groundwater clone 1023 (AB030610) | -------------U---- |
| LKC1006 probe sequence (5′-3′) | CTCCAATGTTTCCATCGG |
| Target sequence | CCGAUGGAAACAUUGGAG |
| Most LKC group II clonesc | ------------------ |
| LKC1.114_5 (AY191480) | ---G-------------- |
| LKC1.199_5 (AY191494) | -U---------------- |
| LKC1.199_6 (AY191495) | ---------U-------- |
a
—, identical to the probe sequence.
b
Based on nine LKC group I clones with 16S rRNA gene sequences at this position.
c
Based on 15 LKC group II clones.