TABLE 1.
Plasmids used in this study
| Plasmid(s) | Relevant characteristicsa | Reference or source |
|---|---|---|
| pANA52 | Apr Kmr, 2.2-kb EcoRI fragment with Ω/Km interposon from pHP45Ω cloned in pUC18 | A. Segura |
| pBBR1MCS-5 | Gmr, oriT RK2 | 11 |
| pHP45Ω-Km | Apr Kmr, Ω/Km interposon | 4 |
| pHP45Ω-Sm | Apr Smr Spr, Ω/Sm interposon | 14 |
| pKNG101 | Smr, mobRK2 oriR6K sacBR | 10 |
| pMC4 | Smr, P. mendocina KR1 tmo/pcu gene cluster cloned at HindIII/BamHI sites in pGV1120 | A. A. Gatenby |
| pMIR15 | Tcr, chimeric cosmid of pLAFR3 carrying P. putida pobA | This study |
| pMIR17 | Apr, 4.5-kb EcoRI/XcaI fragment of pT1-125 carrying the todC1 to todD genes cloned at the EcoRI/SmaI sites of pUC18NB-H | This study |
| pMIR18 | Apr, 6-kb EcoRI/BamHI fragment of pMIR15 cloned in pUC19 | This study |
| pMIR20 | Apr, 1.8-kb SspI/EcoRI fragment of pT1-4, containing the todXF genes, cloned at the EcoRI site of pMIR17 | This study |
| pMIR21 | Apr, todF′ΔtodC1::kilAtelAB::′todC2 | This study |
| pMIR22 | Apr, todF′ΔtodC1::km::′todC2 | This study |
| pMIR27 | Apr Tmr, AT-2 transposon inserted into pobA of pMIR18 | This study |
| pMIR29 | Apr Smr, sacBR todF′ΔtodC1::km::′todC2 | This study |
| pMIR30 | Apr Smr, sacBR todF′ΔtodC1::km::′todC2 | This study |
| pMIR31 | Apr Smr Spr, derivative of pMIR27 with 2-kb SmaI fragment with Ω/Sm interposon at the unique NotI site in AT-2 transposon | This study |
| pMIR32 | Apr, 7.5-kb BamHI fragment of pMC4 containing the tmoXABCDEF genes inserted into pUC19 | This study |
| pMIR40 | Apr, 7.6-kb MluI/NheI fragment of pPCU17 containing the pcuRCAXB genes cloned at the HindII/XbaI sites of pUC18Not | This study |
| pMIR42 | Apr, 7.4-kb BamHI fragment of pMIR32 at the unique BamHI site of pMIR40 | This study |
| pMIR44 | Apr Tcr, 15-kb NotI fragment of pMIR42 with the pcu and tmo gene clusters cloned at the unique NotI site of mini-Tn5Tc of pUT/Tc | This study |
| pPCU17 | Apr, 7.5-kb MluI/NheI containing the pcuRCAXB genes inserted into pSL1180 | A. A. Gatenby |
| pRK600 | Cmr, mob tra | 2 |
| pT1-4 | Apr, 3-kb fragament containing the todXF genes inserted into pUC19 | 13 |
| pT1-125 | Apr, ≈16-kb BamHI fragment that extends from todC1 to todT inserted into pUC19 | 13 |
| pUC18, pUC19 | Apr, MCS, Plac fused to the α peptide of LacZ | 25 |
| pUC18Not | Apr, identical to pUC18 but with NotI polylinker of pUC18-NotI | 8 |
| pUC18NB-H | Apr, identical to pUC18Not but with a deletion from BamHI to HindIII in the polylinker | This study |
| pUT/Tc | Apr Tcr, mini-Tn5Tc inserted into pUT | 2 |
| pUT/Tel | Apr Telr, mini-Tn5kilAtelAB inserted into pUT | 22 |
a
Apr, Cmr, Gmr, Kmr, Smr, Sp, Tcr, Telr, and Tmr, ampicillin, chloramphenicol, gentamicin, kanamycin, streptomycin, spectinomycin, tetracycline, tellurite, and trimethropim resistance, respectively; MCS, multiple cloning site.