TABLE 2.
PCR assays and primers used
| PCR assay | PCR primer | Primer sequence, 5′→3′ | Tm (°C) | PCR product (nt) | Reference |
|---|---|---|---|---|---|
| ERIC | ERIC-1 | ATG TAA GCT CCT GGG GAT TCA C | 60.3 | Random | 18 |
| ERIC-2 | AAG TAA GTG ACT GGG GTG AGC G | 62.1 | |||
| RpoB | RpoB-F | AAC CAG TTC CGC GTT GGC CTG G | 65.8 | 1,088 | 29 |
| RpoB-R | CCT GAA CAA CAC GCT CGG A | 58.8 | |||
| Hsp60 | Hsp60-F | GGT AGA AGA AGG CGT GGT TGC | 61.8 | 341 | |
| Hsp60-R | ATG CAT TCG GTG GTG ATC ATC AG | 60.6 | |||
| HemB | HemB-F | GGC AGA CCA TGA CAG ACT TAA T | 64 | 570 | |
| HemB-R | ACC TGC AGC AGC TGC AAC CA | 60 | |||
| HemBu | HemBu-F | ACT TCT CAC GGT CAC TGC GGT | 61.8 | 459 | |
| HemBu-R | CAT SGC ATA CTC ACC GCT CAC | 61.8 | |||
| HemB_all | HemB_all-F | CG RCG GTT VAG CGG GTT CAT CTG | 66.3 | 237 | |
| HemB_all-R | TGA AYC TBG GCA AGC AGG CBG T | 63.7 | |||
| AmpC | AmpC-F | TCT CTT GCT CTG CTC GCC | 61.4 | 647 | |
| AmpC-R | ACC GCT TTA CCG TCA CGA TAG | 59.8 | |||
| AmpCu | AmpCu-F | AAA TCC CTT TGC TGT GCC CTG | 59.8 | 657 | |
| AmpCu-R | CCA GGC GTA ATG CGC CTC TTC | 63.7 |