TABLE 1.
Effects of butyrate and inulin treatments in piglets1
| Groups
|
P-values
|
||||||
|---|---|---|---|---|---|---|---|
| C | B | I | B + I | B | I | B × I | |
| Cecal fluid | |||||||
| Wet/dry wt | 8.7 ± 0.7 | 7.6 ± 0.8 | 5.9 ± 0.4 | 6.7 ± 1.0 | >0.1 | 0.018 | >0.1 |
| Luminal pH | 7.04 ± 0.11 | 7.01 ± 0.17 | 6.61 ± 0.19 | 6.51 ± 0.13 | >0.1 | 0.007 | >0.1 |
| Cecum | |||||||
| PI L402,3 | 0.21 ± 0.02 | 0.42 ± 0.04* | 0.29 ± 0.03 | 0.29 ± 0.03 | <0.001 | >0.1 | 0.002 |
| ϕh2 | 0.44 ± 0.04 | 0.48 ± 0.03 | 0.42 ± 0.05 | 0.49 ± 0.03 | >0.1 | >0.1 | >0.1 |
| Apoptosis,2% | 0.19 ± 0.07 | 0.27 ± 0.07 | 2.9 ± 2.7 | 2.8 ± 0.1 | >0.1 | >0.1 | 0.077 |
| Distal colon | |||||||
| PI L402,3 | 0.15 ± 0.01 | 0.28 ± 0.02* | 0.19 ± 0.03 | 0.20 ± 0.03 | <0.001 | >0.1 | 0.002 |
| ϕh2 | 0.29 ± 0.04 | 0.26 ± 0.03 | 0.40 ± 0.03 | 0.32 ± 0.04 | >0.1 | >0.1 | >0.1 |
| Apoptosis,2% | 0.07 ± 0.05 | 0.06 ± 0.11 | 0.06 ± 0.06 | 0.04 ± 0.03 | >0.1 | >0.1 | 0.077 |
| Jejunum | |||||||
| Apoptosis,2% | 0.13 ± 0.05 | 0.09 ± 0.04 | 1.30 ± 0.69 | 0.52 ± 0.39 | >0.1 | >0.1 | 0.077 |
| Ileum | |||||||
| Apoptosis,2% | 0.03 ± 0.03 | 2.06 ± 2.06 | 0.0 ± 0.0 | 0.04 ± 0.04 | >0.1 | >0.1 | 0.077 |
1
Values are means ± SEM, n = 6; ileal apoptosis, n = 4.
*
Different from corresponding group without inulin, P < 0.001.
2
Performed as a single analysis including repeated effects for tissue. Inulin × butyrate × tissue interaction, P> 0.1.
3
Lower 40% of the crypt.