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Polymerase chain reaction (PCR) analysis of potential cpn60-1 knockouts. (A) Genetic map of the merozygote region of strain RQL1M-2 showing position of primers cpn60.1F (▸) and cpn60.1R (◂) and sizes of PCR products. (B) PCR analysis. Lanes 1–4: RQL1M-2/pDWcpn.2 KanR SucR GmR; lanes 5–8: RQL1M-2/pTMcpn.3comp KanR SucR GmS; lane 9: DH5α; lane 10: blank (no DNA); lane 11: 1 kb ladder
