Fig 1.

Construction of the N-terminal domain deletion mutant of αB-crystallin (αB-ΔN67). (A) We chose the 67th amino acid as the cleavage site on the basis of the location of the α-crystallin domain (amino acids 68–149). The deletion mutant is named αB-ΔN67. (B) Expression and purification of αB-ΔN67 in Escherichia coli. Full-length αB-crystallin (lane 1) and purified αB-ΔN67 (lane 2) were separated by SDS-PAGE and stained with Coomassie Brilliant Blue. Immunoblotting of full-length αB-crystallin (lane 3) and αB-ΔN67 (lane 4) with anti–αB-crystallin C-terminal peptide antibody