Fig 3.

Tubulin chaperone activity of deletion mutant αB-ΔN67 and full-length αB-crystallin. Kinetics of thermal aggregation with 10 μM tubulin at 42°C alone (closed circle), or in the presence of full-length αB-crystallin (A) or αB-ΔN67 (B) at concentrations of 1.25 μM (open circle), 2.5 μM (closed triangle), and 5 μM (open triangle). Protein aggregation was determined by light scattering at 350 nm. These assays were performed in PME buffer (80 mM PIPES, pH 6.8, 1 mM MgCl₂, 1 mM EGTA, 1 mM GTP)