FIG. 1.
The expression cassettes of the different expression vectors suitable for surface display on S. carnosus together with a schematic picture of the processed gene fusion products, illustrated as anchored to the cell surface. The names of the constructed expression vectors are given below the expression cassettes, and the abbreviated names of the recombinant staphylococci are shown to the right. When a target gene is introduced into the multiple cloning sites of the vector, the encoded target protein is exposed, as illustrated, anchored to the staphylococcal cell surface. M′ represents the processed and covalently anchored form of the M sequence of SpA. Note that the propeptide (PP) from S. hyicus is not processed in S. carnosus. Abbreviations: S, secretion signal from S. hyicus lipase; Bla, gene encoding β-lactamase; Cml, gene encoding chloramphenicol acetyltransferase; OriE, origin of replication from Escherichia coli; OriS, origin of replication from S. aureus; PLip, S. hyicus promoter region designed for S. hyicus lipase production in S. carnosus; X, charged repetitive region postulated to interact with the peptidoglycan cell wall.