FIG. 3.

General procedure for the enrichment of fluorescence-labeled, Z_wt-expressing target cells by flow cytometry. Staphylococcal cells with surface display of affibodies are mixed at the desired ratios and incubated with fluorescence-labeled probes. Target cells are selected on the basis of fluorescence and enriched through multiple rounds of cell sorting and amplification by growth. The cells are plated, and individual clones are screened by PCR for identification.