TABLE 1.

Single-step enrichment of Z_wt-expressing staphylococci at different Z_wt:Z_RSV1 ratios

Zwt:ZRSV1 ratioa	Cell sorting outcome
	% Sc:Zwt cellsb	% Viabilityc	Fold enrichment (theoretical)d
1:1	100	>95	1.6 (1.6)
1:100	95	>95	84 (87)
1:1,000	80	>95	571 (595)

^aBased on optical density measurements of overnight cultures.

^bAs determined by PCR screening of sorted cells by using Z_wt-specific primer pairs.

^cDefined as the percentage of sorted cells capable of growth on chloramphenicol agar plates.

^dTheoretical enrichment factors are based on the actual Z_wt:Z_RSV ratio calculated from FACS analysis of the initial cell populations.