Table 1.
Intracellular protein amount for actin-binding proteins from A. thaliana
Protein | Rosette leaves % of total protein | Suspension cells % of total protein | ABP:actin molar ratio (leaves/susp. cell) |
---|---|---|---|
Actin | 0.36 ± 0.03 (6) | 0.15 ± 0.03 (4) | — |
CAP1 | 0.0593 ± 0.0106 (6) | 0.0554 ± 0.0113 (5) | 1:7/1:3 |
Profilin | 0.32 ± 0.09 (4) | 0.14 ± 0.02 (4) | 3:1/3:1 |
ADF | 0.048 ± 0.005 (4) | 0.07 ± 0.02 (4) | 1:3/1:1 |
Values represent mean percentage ± SD of a particular actin-binding protein with respect to total protein. Rosette leaves were collected at approximately principle growth stage 6 (Boyes et al., 2001). Molar ratios of each ABP to total actin were determined by multiplying the percentage of protein by the ratio of molecular weights and normalizing to actin concentration.