Figure 3.

Protein expression of receptor/ligand pairs. (A) Flow cytometric analysis of BM-MSC surface receptors. Aliquots of cultured BM-MSCs were incubated with FITC- or PE-conjugated monoclonal antibodies against CD45, CD14, CD29, CD49d, CD105, CXCR4, IL-6 receptor α chain, or Sca-1. Cells stained with isotype control IgG conjugated to FITC served as a negative controls (gray peak). Representative results from one of three individual experiments were shown. Values represent percentages of positive cells. (B–D) Immunohistochemical staining for ICAM-1 (B), VCAM-1 (C), and tenascin-C (D). Murine heart sections, 48 h (B and C) and 72 h (D) after MI, were stained with anti-ICAM-1, VCAM-1, or tenascin-C (green) mAb. Myocytes were stained red except in D, and nuclei were stained with blue.