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. 2007 Aug;18(8):2805–2816. doi: 10.1091/mbc.E06-09-0882

Figure 2.

Figure 2.

Ndc80 CEN association is disrupted in spc24-9 mutants. Multiplex PCR analysis of CEN1, PGK1, and CEN3 loci was performed with total chromatin (T) or immunoprecipitate (IP) as PCR templates. Strains were grown at 25°C to log phase and then either shifted to 37°C for 3 h or incubated in 0.2 M HU at 30°C for 3 h. (A) Ndc10-Myc wild type (lanes 1–6) or spc24-9 cells (lanes 7–12). (B) Ndc80-Myc wild-type (lanes 1–6) or spc24-9 cells (lanes 7–12). (C) Wild-type strain carrying no epitope tag (No Tag, lanes 1–6) shown as a control. An untagged spc24-9 mutant was also used as a control and showed similar results (data not shown).