Table 1.
List of primer sequences used in this study (all 60°C annealing)
| Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Product size (bp) |
|---|---|---|---|
| β-Tubulin | GGAACATAGCCGTAAACTGC | TCACTGTGCCTGAACTTACC | 317 |
| Oct-4 | AGAAGGAGCTAGAACAGTTTGC | CGGTTACAGAACCATACTCG | 415 |
| E-cadherin | CGAGAGAGTTACCCTACATA | GTGTTGGGGGCATCATCATC | 214 |
| N-cadherin | CCCAAGTCCAACATTTCCATCC | AAAGCCTCCAGCAAGCACG | 781 |
| Snail | CAGCTGGCCAGGCTCTCGGT | GCGAGGGCCTCCGGAGCA | 843 |
| Slug | CACTCCACTCTCCTTTACC | CAGACTCCTCATGTTTATGC | 597 |
| SIP1 | CGTTCAAACACAAACACC | CCAGTCTCTTCCTCATCC | 454 |
| E12/E47 | GAGGAGTGGCCTCACAAGTGG | GTGCGTGGGACCTTCAGGT | 232 |
| MMP-2 | TGGGTGGAAATTCAGAAGGTGC | ATCTACTTGCTGGACATCAGGGGG | 684 |
| MMP-9 | TGCGACCACATCGAACTTCG | CCAGAGAAGAAGAAAACCCTCTTGG | 686 |
| TIMP-1 | CTTGCATCTCTGGCATCTGG | AAGTAGACAGTGTTCAGGC | 652 |
| TIMP-2 | GAGATCAAGCAGATAAAGATG | GACCCAGTCCATCCAGAGGC | 320 |