Table 2.

Turnover rates calculated from FRAP experiments for the various ligand–receptor interactions at bead contacts

	Transfection
	L1–GFP	L1–GFP	L1–GFPΔCter	NrCAM–GFP
Ligand	L1–Fc	Anti-L1	L1–Fc	TAG-1
koff (h−1)	6.1 ± 1.6	1.0 ± 0.5**	2.2 ± 0.8*	0.18 ± 0.07****
n	24	13	17	8

Data are expressed as mean ± SEM, where n is the number of beads tested. Turnover rates were found to be statistically different by ANOVA and compared with the L1–GFP/L1–Fc condition by Tukey's test (*p = 0.06; **p < 0.01; ****p < 0.001). Data for NrCAM-GFP at TAG-1 bead contacts in B104 neuroblastoma cells were obtained previously (Falk et al., 2004) and are reanalyzed here.