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. 2007 Aug;18(8):3039–3046. doi: 10.1091/mbc.E07-01-0040

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© 2007 by The American Society for Cell Biology

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Figure 4. — Sequential extraction of the glideosome from T. gondii. (A) Diagram of the differential solubilization of Toxoplasma structures during sequential extraction in 1% TX100/PBS (S1 and P1) and 1% TX100/TBS (S2 and P2). (B) Fractions obtained during these extractions were analyzed by immunoblots for the presence of the glideosome subunits (TgMyoA, TgGAP50, TgGAP45, and TgMLC1) and marker proteins for the membrane skeleton (IMC1), plasma membrane (SAG1), endoplasmic reticulum (BiP), dense granules (GRA3), and rhoptries (ROP2). The fractions containing glideosome proteins (P1 and S2) are boxed.