Figure 6.

Sucrose density gradient centrifugation analysis of the TX100/PBS-resistant IMC fraction. Parasites were lysed in 1% TX100 in PBS on ice and the TX100-soluble and -insoluble material was separated by differential centrifugation. The pellet fraction was resuspended, adjusted to 1.6 M sucrose, overlaid with a gradient of 1.4, 1.2, 1.1, 1.0, 0.9, 0.8, 0.7, 0.6, and 0.25 M sucrose and subjected to centrifugation for 2 h at 120,000 × g. Fractions were collected from the bottom of the gradient and analyzed by SDS-PAGE and immunoblotting for IMC1 and TgGAP50.