Figure 7.

Cholesterol is selectively enriched in the Toxoplasma IMC. Parasites were harvested and incubated with no dye (A), BODIPY-CE (B), or BODIPY-PC (C) for 3 h at 37°C. Sequential detergent extractions were performed as described for Figure 4 by using 1% TX100 in PBS (S1 and P1) followed by 1% TX100 in TBS (S2 and P2), both on ice. Lipids were extracted from all fractions and analyzed by TLC. The extraction efficiency of TgGAP50 in these buffers was confirmed by immunoblot analysis. STD, lipid standard.