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The TX100/PBS-resistant cholesterol and TgGAP50 in the IMC are efficiently extracted with TX100 in TBS. (A) BODIPY-cholesteryl ester accumulates in the Toxoplasma IMC, as judged by the overlap with GAP50. IMC-associated BODIPY-CE is resistant to extraction by TX100/PBS but is solubilized by TX100/TBS. (B) BODIPY-PC is found throughout the parasite and is completely extracted in TX100/PBS. P1 and P2 refer to extraction performed as in Figure 4. Bar, 5 μm.
