Figure 3.

Kif2b is essential for spindle bipolarity. (A) Untreated U2OS or U2OS cells transfected with Kif2b-specific siRNA were fixed and stained for microtubules in green and DNA in blue. Scale bar, 5 μm. (B) Quantification of the percentage of mitotic cells with bipolar spindles after transfection with a control siRNA (untreated) or Kif2b-specific siRNA. Error bars, SD. (C) Total cell extracts prepared from GFP-Kif2b expressing U2OS cells that were either untreated or transfected with Kif2b-specific siRNA were separated by size by SDS-PAGE and blotted with antibodies specific to GFP and actin as indicated.