Figure 5.

Kif2b-deficient cells form stable kinetochore microtubule attachments. U2OS cells were either untreated or transfected with Kif2b-specific siRNA as indicated. Cells were extracted in buffer containing 1 mM calcium for 4 min before fixation to induce depolymerization of nonstable microtubules. Microtubules are shown in green, DNA in blue, and kinetochores (Hec1 staining) in red. The insets in the top right corners of each panel are 3× magnifications of kinetochore pairs (white box). Scale bar, 5 μm.