Figure 3.

Flux contributes to the anaphase A segregation rate of chromosomes. (A) Measurement of flux and segregation rates in an anaphase spindle. Pre, prebleached spindle; microtubules, green; chromatin, red. Numbers are elapsed time (seconds) after photobleaching. The blue arrow marks the progression of the photobleach mark toward the pole as a result of flux. (The dashed yellow line marks the end of a pole and, coincidentally, the center of a centrosome.) The yellow arrow marks the leading edge of a selected chromosome as it moves poleward. Scale bar, 2 μm. (B) Average anaphase flux rates after the indicated RNAi treatments. (C) Average anaphase chromatid-to-pole velocities after RNAi treatment.