Figure 2.

dnt1Δ promotes proper completion of cytokinesis in the cdc14-118 myo2-E1 cells. Asynchronous cells of the indicated genotypes were grown at 25°C to log phase cell density, and portions were shifted to 30°C for 4 h. Cells were methanol fixed and stained simultaneously with DAPI and calcofluor to score for the number of nuclei per cell (A) and septum formation (B), respectively. Cells were scored for the presence of normal, aberrant, or lack of septa between nuclei. At least 100 cells were examined for each strain, and mean values (n = 3) were plotted.