Figure 6.

(A) Suppressors do not cause premature release of Clp1 from nucleolus. Wild-type and mutant cells with indicated genotypes were first grown at 25°C, and then they were shifted to 30 or 36°C for 4 h before being fixed and stained with DAPI. Arrows indicate the nuclei enlarged in insets. (B) Cells of the indicated genotypes were grown to log phase at 25°C, and then they were shifted to 30°C for 4 h. These cells were then methanol fixed and imaged for Clp1-GFP localization. The ratio of the mean average intensity of the Clp1-GFP signal in the nucleolus and cytoplasm is shown (n > 50). Representative images are shown. Only cells that were in telophase, as judged by nuclear positioning, were analyzed.