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The ArfGAPs Glo3p and Gcs1p mediate binding of Arf1ΔN17p-Q71L to the cytoplasmic domains of SNAREs. SNARE–GST fusion proteins were immobilized onto glutathione-agarose beads and incubated with Arf1ΔN17p-Q71L. Where indicated Glo3p (A and C) or Gcs1p (B) was added to the reaction. Bound proteins were separated by SDS-PAGE, and bands were visualized by Coomassie blue staining.
