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Gcs1p mediates Sec18p recruitment to microsomal membranes. Buffer-washed microsomal membranes were incubated with His6-Sec18p and ATP in the absence (lane 2) or presence (lane 3) of Gcs1p. After extensive washing, the membrane fraction was solubilized and analyzed by immunoblot. Lane 1 contains the microsomal membranes incubated with buffer. Ten percent of the Gcs1p and 2% of Sec18p present in the incubation are shown in lanes 4 and 5, respectively.
