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Specificity of the PDILT antiserum. (A) Lysates from HeLa cells transfected with PDILT-myc or an irrelevant myc construct were analyzed by Western blotting with a rabbit antiserum raised against purified recombinant His-tagged human PDILT. (B) Equal amounts (in mg of tissue) of murine tissue lysates were analyzed by Western blotting with a rabbit antiserum raised against purified recombinant His-tagged human PDILT (top), anti-PDI (middle), and anti-calnexin (bottom).
