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. 2007 Aug;18(8):2795–2804. doi: 10.1091/mbc.E07-02-0147

Figure 5.

Figure 5.

Expression of PDILT commences during the first wave of spermatogenesis. (A) Total RNA extracted from testis of 2-, 15-, 30-, 45-, and 58-d-old rats was used for RT-PCR using primer sets specific for PDILT, calmegin, PDI, and actin. As a positive control, cDNA was used for PDILT, calmegin, and PDI. PDILT primer set 1 was designed on 2 internal exons, whereas PDILT primer set 2 was designed on the first and last exons, to exclude expression of alternatively spliced variants during development. (B) Testis and liver from 2-, 15-, 30-, 45-, and 58-d-old rats were lysed and analyzed by Western blotting for PDILT, calmegin, and PDI. (C) Testis sections from 21-, 28-, 31-, 38-, 45-d-old and adult rats were counterstained with hematoxylin (blue) and immunostained for PDILT (brown).