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PDILT self-associates. Wild-type (wt) or cysteine mutant (C135A/C420A, denoted CA) HA- or myc-tagged PDILT was expressed in HeLa cells as indicated. Lysates were directly analyzed (lanes 1–4) or subjected to immunoprecipitation with anti-myc (lanes 5–8) or anti-HA (lanes 9–12) before SDS-PAGE and Western blotting with anti-HA (bottom panel) and anti-myc (top panel). The asterisk indicates the bands for the antibodies used for the immunoprecipitations.
