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. 2007 Aug;18(8):3015–3025. doi: 10.1091/mbc.E06-09-0869

Figure 1.

Figure 1.

Effect of BFA on uptake and storage of oleate. (A) NRK cells were continuously grown in the presence of 2 μg/ml BFA plus 100 μM oleate for the indicated time. These images are taken from the movie supplied in supplementary data. (B) CHO K2 cells were incubated in the presence of either 2 μg/ml BFA or EtOH carrier for the indicated time. Droplet-enriched fractions were prepared, equal amounts of protein separated on 12% SDS-PAGE and immunoblotted with α-ADRP and α-cyclophilin A IgG (loading control). (C) CHO cells were incubated in the presence of 2 μg/ml BFA (BFA) or EtOH carrier (control) for 12 h. Cells were fixed and processed to visualize neutral lipids with oil red O. Bar, 10 μm.