Figure 8.

CtBP/BARS-deficient MEFs are defective in lipid storage. Mouse embryo fibroblasts (WT and CtBP1/BARS1/2^−/−) were cultured as described in text. (A) One set of cells was incubated in the presence of 50 μCi of [³H]oleate] for 24 h, washed, and processed to extract lipids as described in text. An equal volume of lipid extract from the two cells was separated by TLC, and then it was processed for autoradiography (left). In a separate experiment (right), equal volumes of lipid extract from the two cell lines were separated, scraped, and counted in a scintillation counter. The result is the average of three samples plus the SD. (B) WT and KO fibroblasts were grown as described in text. Cells were fixed and stained with α-CtBP1/BARS IgG, and the nucleus was visualized with Hoechst dye and the neutral lipids with oil red O. Bar, 10 μm.