Figure 9.

Loss of parkin unmasks native ASIC current potentiation in hippocampal neurons. (A) Representative native ASIC inward currents evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after OAG (50 μM, 30 min) in hippocampal neurons cultured from parkin wild-type, heterozygous, and knockout mice. (B) ASIC currents in parkin knockout neurons mice showed strong OAG-induced potentiation (+176%), whereas heterozygotes showed an intermediate albeit nonsignificant trend toward potentiation (+46%). In contrast, OAG treatment did not potentiate ASIC currents in wild-type neurons (peak currents evoked by pH 5.0, OAG: 906 ± 211 pA; vehicle: 830 ± 208 pA). The data are expressed as mean ± SEM of normalized currents (n = 5–10). **p < 0.01, Student's t test.