Figure 4.

AML1/ETO transactivates the BCL-2 promoter. U937 cells were cotransfected with pGL2-basic or pGL2-BCL-2 reporter gene constructs and with pCMV-AML1A, pCMV-AML1B or pCMV-AML1/ETO expression vectors (together with pGHX5 to normalize transfection efficiency). Promoter activity was measured as the ratio of the luciferase activity and the concentration of growth hormone in the conditioned media. The promoter activity in the presence of the empty CMV5 plasmid was defined as 1 and the promoter activity in the presence of expression vectors for AML1A, AML1B, and AML1/ETO was defined relative to that value. The results shown are the mean of four different experiments (SEs are shown when measurable).