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The AML1 binding site in the SacI fragment confers AML1/ETO responsiveness to a heterologous promoter. U937 cells were cotransfected with the pTE2, pTE2/SacI or pTE2/SacIm plasmids and with AML1/ETO expression vector (AML1/ETO sense), with its antisense derivative (AML1/ETO antisense) or with an empty pCIneo vector (0). The activity of the pTE2 plasmid in the presence of an empty vector was assigned a value of one. Results from one of three independent experiments are shown.
