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. 2003 Sep;69(9):5170–5177. doi: 10.1128/AEM.69.9.5170-5177.2003

FIG. 2.

FIG. 2.

PCR amplification of alkB from spring, summer, fall, and winter biofilms with (CNP) and without (Con) nutrient amendments. Total-community DNA extracts were undiluted (0) or diluted 10−1 (−1) or 10−2 (−2) prior to PCR amplification using the alkB consensus primers. Lanes M, molecular size markers; lane +, positive control (50 ng of Pseudomonas oleovorans DNA template); lane −, negative control (no DNA). The arrows indicate the positions of the 549-bp alkB gene amplicon, and the asterisks indicate the positions of the 500-bp fragments of the molecular size markers.