TABLE 3.
Concentrations of DNA extracted from cysts and oocysts following modifications to the DNeasy DNA extraction method
| DNA extraction modificationa | Concn of DNA (ng/1.5 × 105 cysts or oocysts)b
|
|
|---|---|---|
| G. lamblia cysts | C. parvum oocysts | |
| 1 h at 55°C + F/T | 5.89 ± 1.76 | 6.21 ± 0.83 |
| 1 h at 55°C + F/T + sonication (probe) | 24.28 ± 2.89 | 10.22 ± 1.52 |
| 1 h at 55°C + F/T + sonication (cup horn) | 24.62 ± 3.42 | 13.59 ± 0.64 |
| F/T + sonication (cup horn) | 29.48 ± 2.52 | 16.60 ± 1.14 |
a
F/T, three cycles of freeze-thaw.
b
Data are presented as the mean ± standard deviation of triplicate extractions per treatment group.