TABLE 5.
Effects of Chelex 100 and PVP 360 on multiplex qPCR detection of Giardia and Cryptosporidium DNAs spiked into lake water extracts
| Water samplea | Ctb
|
|
|---|---|---|
| β-Giardin P241 | COWP P702 | |
| ddH2O | 24.88 ± 0.69 | 27.36 ± 0.40 |
| Heart Lake | ||
| Untreated | 23.98 ± 0.09 | 27.09 ± 0.35 |
| Treatedc | 24.34 ± 0.89 | 26.70 ± 0.89 |
| Professor's Lake | ||
| Untreated | None | None |
| Treated 1c | 24.89 ± 0.13 | 27.61 ± 0.19 |
| Treated 2c | 25.15 ± 0.94 | 27.99 ± 0.60 |
a
2.5 μl from 50 μl of each sample extract was assayed.
b
Mean ± standard deviation of triplicate wells.
c
Treated samples had 0.5 M EDTA, 20% Chelex 100, and 2% PVP 360 treatment during DNA extraction.