TABLE 6.
Multiplex qPCR detection of 1 ng of spiked DNA to determine the presence of inhibitors in 1-liter sewage sample extracts
| Sample | Vol added to qPCR assay (μl) | Ctb
|
|
|---|---|---|---|
| β-Giardin P241 | COWP P702 | ||
| ddH2O | 10 | 23.30 ± 0.63 | 23.88 ± 0.25 |
| Extraction control 1a | |||
| 10 | 23.23 ± 0.23 | 23.12 ± 0.29 | |
| 1 | 23.31 ± 0.49 | 23.63 ± 0.55 | |
| Extraction control 2a | |||
| 10 | 23.95 ± 0.54 | 23.30 ± 0.27 | |
| 1 | 23.31 ± 0.73 | 23.51 ± 0.41 | |
| Auteuil A1 | |||
| 10 | None | None | |
| 1 | 24.20 ± 0.31 | 24.12 ± 0.26 | |
| Auteuil A2 | |||
| 10 | 26.37 ± 0.61 | 25.00 ± 0.55 | |
| 1 | 23.51 ± 0.70 | 23.52 ± 0.46 | |
a
Extraction controls, all DNA extraction methods minus sewage.
b
Mean ± standard deviation of triplicate wells.