FIG. 3.

Alignment showing the targeting region of probe pmoA636b among pmoA2 and corresponding pmoA1 sequences of those MOB investigated by Southern hybridization. The clone M84-P3 is shown as a representative of a set of diverse environmental pmoA2 sequences that perfectly match in the target region probe pmoA636b, including clones RsVc-03, Rr90a-43, RsVc-07, Rz90f-09, RsVc-05, and Rr90a-01 (Fig. 1). Letters shown in boldface indicate weak G:T mismatching nucleotide positions, while those shown in boldface and underlined indicate stronger mismatches (e.g., C:T, C:C, or G:G). Strains shown in the alignment are Methylocystis strain SC2, M. trichosporium strains OB3b and SM6, Methylomonas strain D1a, Methylocaldum strain E10a, and M. capsulatus strain Bath.