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. 2003 Sep;69(9):5593–5602. doi: 10.1128/AEM.69.9.5593-5602.2003

FIG. 4.

FIG. 4.

Southern hybridization of genomic DNA to oligonucleotide probe pmoA636b (A lanes) or PCR-generated universal pmoA gene probe (B lanes). Under the hybridization conditions used in this study, probe pmoA636b is specific for pmoA2 (Fig. 3), while the universal pmoA gene probe detects both pmoA1 and pmoA2. (I) EcoRI digests. Lanes: 1, M. trichosporium strain OB3b; 2, Methylocaldum strain E10A; 3, Methylocystis strain SC2. (II) XhoI digests. Lanes: 4, Methylosinus trichosporium strain SM6; 5, Methylocystis strain SC2. (III) PstI digests. Lanes: 6, Methylococcus capsulatus strain Bath; 7, Methylocystis strain SC2; 8, Methylomonas strain D1a. Methylocystis strain SC2 was used as positive control (9). The PstI digest of DNA of phage λ was used as a size marker.