FIG. 1.

Genotyping of Cryptosporidium isolates in reclaimed effluents with the small-subunit rRNA-based PCR-restriction fragment length polymorphism technique. Differentiation of Cryptosporidium genotypes was demonstrated by digestion of the secondary PCR product with VspI. Lane 1, no sample; lanes 2 and 5, C. parvum bovine genotype; lanes 3 and 4, C. parvum human and bovine genotypes; lanes 6 and 7, C. parvum human genotype; and lane M, 100-bp DNA ladder. Upper and lower arrows correspond to band sizes of 600 and 100 bp, respectively.